무막줄기세포추출물의 3T3-L1 세포에서 포도당 흡수 촉진 효과

• 영문명: Membrane Free Stem Cell Extract from Adipose Tissue Enhances Glucose Uptake in 3T3-L1 Cells
• 발행기관: 한방비만학회
• 저자명: 김지현(Ji Hyun Kim) 김민정(Min Jeong Kim) 박혜숙(Hye Sook Park) 김영실(Young Sil Kim) 조은주(Eun Ju Cho)
• 간행물 정보: 『대한한방비만학회지』제19권 2호, 89~96쪽, 전체 8쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2019.12.31

영문 초록

Objectives: We investigated whether membrane free stem cell extract from adipose tissue (MFSCE) has anti-diabetic effect. Methods: To determine glucose uptake effect of MFSCE, we carried out glucose uptake assay in 3T3-L1 adipocytes. The regulatory mechanisms of MFSCE on glucose uptake were examined by Western blot analysis. Results: When MFSCE was treated to adipocytes at the concentration of 0.5, 1, 2.5, and 5 μg/mL, 2-deoxyglucose-6-phosphate uptake was elevated approximately 1.8-fold compared to cells not treated with MFSCE. It indicated that MFSCE enhances glucose uptake in 3T3-L1 adipocytes. In addition, MFSCE reduced phosphorylation of insulin receptor substrate-1 at serine 307 and induced Akt and glucose transporter 4 protein expressions that were related to insulin signaling. Furthermore, MFSCE regulated adenosine monophosphate-activated protein kinase (AMPK) pathway by increases of increase phosphorylation of AMPK and acetyl-CoA carboxylase that were related to AMPK pathway. Conclusions: These results indicated that MFSCE promotes glucose uptake via modulation of insulin signaling and AMPK pathway. Therefore, MFSCE could be a promising agent for treatment of diabetes mellitus.

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키워드

AMP-activated protein kinases Diabetes mellitus Membrane free stem cell extract 3T3-L1 cells