A transcription factor OsNAC075 is essential for salt resistance in rice(Oryza sativa L.)

• 발행기관: (사)한국식물생명공학회
• 저자명: Yu Jin Jung Myung Chul Lee Kwon Kyoo Kang
• 간행물 정보: 『Journal of Plant Biotechnology』38권 1호, 94~104쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.03.31

영문 초록

Salt stress is a major environmental factor influencing plant growth and development. To identify salt tolerance determinants, we systematically screened salt sensitive rice mutants by use of the Activator/Dissociation (Ac/Ds) transposon tagging system. In this study, we focused on the salt sensitive mutant line, designated SSM-1. A gene encoding a NAC ranscription factor homologue was disrupted by the insertion of a Ds ransposon into SSM-1 line. The OsNAC075 gene (EU541472) has 7 exons and encodes a protein (486-aa) containing the NAC domain in its N-terminal region. Sequence comparison showed that the OsNAC075 protein had a strikingly conserved region at the N-terminus, which is considered as the characteristic of the NAC protein family. OsNAC075 protein was orthologous to Arabidopsis thaliana ANAC075. Phylogenetic analysis confirmed OsNAC075 belonged to the OsNAC3 subfamily, which plays an important role in response to stress stimuli. RT-PCR analysis showed that the expression of OsNAC075 gene was rapidly and strongly induced by stresses such as NaCl, ABA and low temperature (4℃). Our data suggest that OsNAC075 holds promising utility in improving salt tolerance in rice.

키워드

Ds insertion mutant OsNAC075 rice salt stress transcription factor