안정적 감자 엽록체 형질전환 식물체 생산

• 영문명: Production of stable chloroplast-transformed plants in potato (Solanum tuberosum L.)
• 발행기관: (사)한국식물생명공학회
• 저자명: 민성란(Sung Ran Min) 정원중(Won Joong Jeong) 박지현(Ji Hyun Park) 유재일(Jae Il Lyu) 이정희(Jeong Hee Lee) 오광훈(Kwan
• 간행물 정보: 『Journal of Plant Biotechnology』38권 1호, 42~48쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.03.31

영문 초록

Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast ransformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.