잎의 발달단계의 Leaf index를 조절하는 애기장대 MACROPHYLLA / ROTUNDIFOLIA3 유전자

• 영문명: MACROPHYLLA/ROTUNDIFOLIA3 gene of Arabidopsis controls Leaf index during Leaf development
• 발행기관: (사)한국식물생명공학회
• 저자명: 전상은(Sang Eun Jun) 투말라찬드라쉐이커(Thummala Chandrasekhar) 조규형(Kiu Hyung Cho) 이영병(Young Byung Yi) 형남인(Nam I
• 간행물 정보: 『Journal of Plant Biotechnology』38권 4호, 285~292쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.12.31

영문 초록

In plants, heteroblasty reflects the morphological adaptation during leaf development according to the external environmental condition and affects the final shape and size of organ. Among parameters displaying heteroblasty, leaf index is an important and typical one to represent the shape and size of simple leaves. Leaf index factor is eventually determined by cell proliferation and cell expansion in leaf blades. Although several regulators and their mechanisms controlling the cell division and cell expansion in leaf development have been studied, it does not fully provide a blueprint of organ formation and morphogenesis during environmental changes. To investigate genes and their mechanisms controlling leaf index during leaf development, we carried out molecular-genetic and physiological experiments using an Arabidopsis mutant. In this study, we identified macrophylla (mac) which had enlarged leaves. In detail, the mac mutant showed alteration in leaf index and cell expansion in direction of width and length, resulting in not only modification of leaf shape but also disruption of heteroblasty. Molecular-genetic studies indicated that mac mutant had point mutation in ROTUDJFOLIA3 (ROT3) gene involved in brassinosteroid biosynthesis and was an allele of rod-I mutant. We named it rnac/rot3-5 mutant. The expression of ROT3 gene was controlled by negative feedback inhibition by the treatment of brassinosteroid hormone, suggesting that ROT3 gene was involved in brassinosteroid biosynthesis. in dark condition, in addition, the expression of ROT3 gene was up-regulated and mac/rot3-5 mutant showed lower response, compare to wild type in petiole elongation. This study suggests that ROT3 gene has an important role in control of leaf index during leaf expansion process for proper environmental adaptation, such as shade avoidance syndrome, via the control of brassinosteroid biosynthesis.

키워드

Brassinosteroid Heteroblasty Leaf index macrophylla Shade avoidance