동백나무 미숙배 배양으로부터 비정상 체세포배 형성과 식물체 재생

• 영문명: Anomalous somatic embryos formation and plant regeneration from the cultures of immature embryos of Camellia japonica L.
• 발행기관: (사)한국식물생명공학회
• 저자명: 최종혜(Jong Hye Choi) 권석윤(Suk Yoon Kwon) 최필선(Pil Son Choi)
• 간행물 정보: 『Journal of Plant Biotechnology』38권 4호, 258~262쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.12.31

영문 초록

Embryogenic callus was induced from the cultures of immature embryos of Camellia japonica L. on Murashige & Skoog`s (MS) solid medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D), and then the embryogenic callus was proliferated on same medium for 4 weeks over. The embryogenic callus was sub-cultured on MS basal medium without 2,4-D to produce coyledonary stage of somatic embryo. The frequency (%) of somatic embryogenesis was 25.1%, and the majority of somatic embryos formed had a abnormal morphology with cupshaped cotyledon (48.3%), one cotyledon (12.6%), three cotyledons (9.4%), four cotyledons (1.9%), whereas was only normal morphology with two cotyledon (27.5%). When the somatic embryos with normal or abnormal cotyledons transfer to MS basal medium or ½ MS medium with/or without plant growth regulators (GA3, IBA) for regeneration, the frequency (%) of two-cotyledon embryos regenerated into plantlets was higher 11.1% than one cotyledon (0.0~8.3 %), three cotyledons (0.0~5.8%), four cotyledons (0.0%), cup-shaped (0.3~4.2%). These results demonstrated that the anomalous cotyledons of somatic embryos could caused to decrease the rate of plant regeneration.

키워드

Abnormal cotyledon plant regeneration somatic embryos