벼에서 CRISPR/Cas9 활용 고빈도 유전자 편집 방법

• 영문명: A novel method for high-frequency genome editing in rice, using the CRISPR/Cas9 system
• 발행기관: (사)한국식물생명공학회
• 저자명: 정유진(Yu Jin Jung) 배상수(Sangsu Bae) 이긍주(Geung-joo Lee) 서필준(Pil Joon Seo) 조용구(Yong-gu Cho) 강권규(Kwon Kyoo
• 간행물 정보: 『Journal of Plant Biotechnology』44권 1호, 89~96쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.03.31

영문 초록

The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritabletargeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

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키워드

CRISPR/Cas9 Gene editing sgRNA design transgenic rice null plant