현사시나무에서 Dormancy-associated protein 1 (DRM1) 유전자의 분리와 발현 특성 구명

• 영문명: Isolation and Expression of Dormancy-associated protein 1 (DRM1) in Poplar (Populus alba × P. glandulosa)
• 발행기관: (사)한국식물생명공학회
• 저자명: 윤서경(Seo-kyung Yoon) 배은경(Eun-kyung Bae) 최현모(Hyunmo Choi) 최영임(Young-im Choi) 이효신(Hyoshin Lee)
• 간행물 정보: 『Journal of Plant Biotechnology』44권 1호, 69~75쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.03.31

영문 초록

Dormancy-associated protein (DRM) is involved in the dormancy physiology of plants and is conserved in almost all plant species. Recent studies found that DRM genes are involved in the abiotic stress response, and characterization studies of these genes have been conducted in several plants. However, few studies have focused on DRM genes in woody plants. Therefore, in this study, cDNA coding for DRM (PagDRM1) was isolated from poplar (Populus alba × P. glandulosa), and its structure and expression characteristics were investigated. PagDRM1 encodes a putative protein composed of 123 amino acids, and the protein contains two conserved domains (Domain I and Domain II). PagDRM1 is present as one or two copies in the poplar genome. Its expression level was highest in the stem, followed by mature leaves, roots, and flowers. During the growth of cultured cells in suspension, PagDRM1 was highly expressed from the late-exponential phase to the stationary phase. In addition, PagDRM1 expression increased in response to drought, salt stress, and treatment with plant hormones (e.g., abscisic acid and gibberellic acid). Therefore, we suggested that PagDRM1 not only plays an important role in the induction of dormancy, but also contributes to stress tolerance in plants.

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키워드

Abiotic stress Dormancy Gene expression PagDRM1 Populus alba×P.glandulosa