Overexpression of Brassica rapa GROWTH-REGULATING FACTOR genes in Arabidopsis thaliana increases organ growth by enhancing cell proliferation

• 발행기관: (사)한국식물생명공학회
• 저자명: Joon Ki Hong Seon-Woo Oh Jeong Hoe Kim Seung Bum Lee Eun Jung Suh Yeon-Hee Lee
• 간행물 정보: 『Journal of Plant Biotechnology』44권 3호, 271~286쪽, 전체 16쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2017.09.30

영문 초록

GROWTH-REGULATING FACTOR (GRF) genes encode plant-specific transcription factors containing two conserved QLQ and WRC domains and play critical roles in regulating the growth and development of lateral organs, such as cotyledons, leaves, and flowers. To explore the agricultural potential of Brassica rapa GRF genes (BrGRFs), the researchers isolated seven BrGRFs (BrGRF3-1, 3-2, 5, 7, 8-1, 8-2, and 9) and constructed BrGRF-overexpressing Arabidopsis thaliana plants (BrGRF-OX). BrGRF-OX plants developed larger cotyledons, leaves, and flowers as well as longer roots than the wild type. The increase in size of these organs were due to increases in cell number, but not due to cell size. BrGRF-OX plants also had larger siliques and seeds. Furthermore, BrGRF-OX seeds produced more oil than the wild type. RT-PCR analysis revealed that BrGRFs regulated expression of a wide range of genes that are involved in gibberellin-, auxin-, cell division-related growth processes. Taken together, the data indicates that BrGRFs act as positive regulators of plant growth, thus raising the possibility that they may serve as a useful genetic source for crop improvement with respect to organ size and seed oil production.

목차

Introduction

Material and Methods

Results and Discussion

키워드

Cell division GROWTH-REGULATING FACTOR Morphogenesis Organ size Seed oil Transcription factor