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• 발행기관: 한국축산식품학회
• 저자명: 김명희 김왕준 신원선 손동화 차성관
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제23권 제3호, 278~283쪽, 전체 6쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2003.09.30

영문 초록

Feasibility tests on using liposomes for detecting food-borne pathogenic bacteria were studies with E. coli O157:H7 as a model analyte. Immunoliposomes, whose surface was conjugated with anti-E. coli O157:H7 lgG and which encapsulated the marker dye, sulforhodamine B, were used for the detection label. Among the feasibility tests, the first test was to use a test-strip on which antibodies to anti-E. coli O157:H7 lgG were immobilized. In this format, immunolipsosmes that did not bind to E. coli O157:H7 in sample were captured and then exhibited a visible signal which was inversely related with the number of E. coli O157:H7 in sample. The second test was a direct liposome assay followed by immunomagnetic separation. In this format, immunoliposomes which were bound to E. coli O157:H7 were lysed with detergent and produced a signal which was proportionally related with the number of E. coli O157:H7 in sample. The results from both formats indicate that liposomes can be utilized as a detection label.

키워드

liposome test-strip immunomagnetic bead E. coli O157:H7 immunoassay safety