Modulation of Large Conductance Ca2⁢-activated K⁢ Channel of Skin Fibroblast (CRL-1474) by Cyclic Nucleotides

• 영문명: Modulation of Large Conductance Ca^2⁢-activated K^⁢ Channel of Skin Fibroblast (CRL-1474) by Cyclic Nucleotides
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Jihyun Yun Seungtae Kim Hyoweon Bang
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제9권 제2호, 131~134쪽, 전체 4쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2005.01.01

영문 초록

Potassium channels in human skin fibroblast have been studied as a possible site of Alzheimer disease pathogenesis. Fibroblasts in Alzheimer disease show alterations in signal transduction pathway such as changes in Ca^2⁢ homeostasis and/or Ca^2⁢-activated kinases, phosphatidylinositol cascade, protein kinase C activity, cAMP levels and absence of specific K^⁢ channel. However, little is known so far about electrophysiological and pharmacological characteristics of large-conductance Ca^2⁢-activated K^⁢ (BK_Ca) channel in human fibroblast (CRL-1474). In the present study, we found Iberiotoxin- and TEA-sensitive outward rectifying oscillatory current with whole-cell recordings. Single channel analysis showed large conductance K^⁢ channels (106 pS of chord conductance at ⁢40 mV in physiological K^⁢ gradient). The 106 pS channels were activated by membrane potential and [Ca^2⁢]_i, consistent with the known properties of BK_Ca channels. BK_Ca channels in CRL-1474 were positively regulated by adenylate cyclase activator (10μM forskolin), 8-Br-cyclic AMP (300μM) or 8-Br-cyclic GMP (300μM). These results suggest that human skin fibroblasts (CR-1474) have typical BK_Ca channel and this channel could be modulated by c-AMP and c-GMP. The electrophysiological characteristics of fibroblasts might be used as the diagnostic clues for Alzheimer disease.

키워드

BK_Ca channel Fibroblast Alzheimer disease Second messenger system cAMP cGMP