Interactions between Estradiol-17β-BSA and Calcitropic Hormones in Ca2⁢ Uptake in Renal Proximal Tubule Cells

• 영문명: Interactions between Estradiol-17β-BSA and Calcitropic Hormones in Ca^2⁢ Uptake in Renal Proximal Tubule Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Ho Jae Han Yeun Hee Lee Eun Ju Seo
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제6권 제5호, 261~268쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2002.01.01

영문 초록

The aim of the present study was to investigate the interaction of estradiol-17β-bovine serum albumin (E₂-BSA) and calcitropic hormones, such as parathyroid hormone, calcitonin, and vitamin D, in regulation of Ca^2⁢ uptake in primary cultured renal proximal tubule cells. Statistically significant increase in Ca^2⁢ uptake was found from 2 hours after E₂-BSA (10^⁣9 M) treatment, while estradiol-17β (10^⁣9 M) did not affect. Treatment of the cells with E₂-BSA (10^⁣9 M) together with parathyroid hormone (PTH) (10^⁣8 M), vitamin D (10^⁣8 M), or calcitonin (10^⁣8 M) significantly stimulated Ca^2⁢ uptake by 32.50%, 29.30%, or 27.75%, respectively, compared with the control. However, calcitropic hormones did not exhibit any synergistic effect on the E₂-BSA-induced stimulation. E₂-BSA significantly increased cAMP generation and PKC activity. The stimulatory effect of cotreatment of E₂-BSA and PTH or vitamin D was blocked by SQ22536 (an adenylate cyclase inhibitor) and staurosporine (a PKC inhibitor), but the effect of cotreatment of E₂-BSA and calcitonin was not blocked. Furthermore, 8-Br-cAMP and TPA (an artificial PKC promoter) increased Ca^2⁢ uptake by 25.51% and 16.47%, respectively, compared with the control. In conclusion, E₂-BSA combined with calcitropic hormones regulated Ca^2⁢ uptake partially via cAMP and PKC-dependent mechanisms in renal proximal tubule cells.

키워드

E₂-BSA Ca^2⁢ uptake PTH vitamin D calcitonin cAMP PKC