C2C12 골격근세포에서 황기의 미토콘드리아 조절 작용

• 영문명: Original Article : The Effects of Astragali Radix Extracts on Mitochondrial Function in C2C12 Myotubes
• 발행기관: 한방비만학회
• 저자명: 송미영(Mi Young Song)
• 간행물 정보: 『대한한방비만학회지』제14권 2호, 55~62쪽, 전체 8쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2014.12.31

영문 초록

Objective: The prevalence of metabolic syndrome and type 2 diabetes is increasing worldwide. Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researches have been increasing highly. Astragali Radix extract (ARE) or its main components have been shown to perform comparably to insulin by significantly reducing blood glucose levels in animalmodels however, the influence on mitochondrial dysfunction are not well understood. Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6day-differentiation. The expressions of adenosine monophosphate (AMP)-activated proteinkinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror γcoactivator 1α (PGC1α), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor(Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reactionanalysis. The morphological changes were also investigated. Results: ARE dose dependently increased phosphorylation of AMPK and respectively activated mRNA expressions of PGC1α, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between ARE treatment and control. Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrialfunction by activating AMPK and PGC1α.

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키워드

Astragali radix extract Mitochondria Myotube AMP-activated protein kinases Proliferators-activated receptror γ coactivator 1α