AlphaFold3-predicted models of the endospore coat protein SpoIVA

• 발행기관: 한국구조생물학회
• 저자명: Doyeon Kim Nam-Chul Ha
• 간행물 정보: 『Biodesign』Vol 12, No 4, Dec, 63~69쪽, 전체 7쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2024.12.31

국문 초록

Bacillus cereus is a Gram-positive, aerobic bacterium that forms highly resilient endospores encased in a multilayered protective coat. Within the endospore, SpoIVA is localized to the basement layer of the spore coat via the direct interaction with the basement-localized protein SpoVM. In this study, we employed AlphaFold3, an AI-powered structural prediction program, to model the 3D structures of SpoIVA’s functional oligomers and to predict the complex structure of SpoIVA bound to SpoVM. Our findings identified a Y-shaped symmetric structure of the SpoIVA dimer, stabilized by interactions within its C-terminal domain, with SpoVM binding specifically on the C-terminal region of SpoIVA. AlphaFold3 further predicted an ATP-dependent oligomerization structure associated with the N-terminal domain of SpoIVA. The MD simulation suggested the dynamic structural nature of the SpoIVA oligomers. These results deepen our understanding of the structural and functional relationships among these spore-forming proteins, offering new insights into their roles in endospore architecture.

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INTRODUCTION

RESULTS

DISCUSSION

MATERIALS AND METHODS

ACKNOWLEDGEMENTS

CONFLICT OF INTEREST

REFERENCES