Development and Utilization of KASP Markers Targeting the Lipoxygenase Gene in Soybean

• 영문명: Development and Utilization of KASP Markers Targeting the Lipoxygenase Gene in Soybean
• 발행기관: 한국작물학회
• 저자명: 신서영(Seo-Young Shin) 강세희(Se-Hee Kang) 강병희(Byeong Hee Kang) Sreeparna Chowdhury 이원호(Won-Ho Lee) 이정동(Jeong-Dong Lee) 이성우(Sungwoo Lee) 최유미(Yu-Mi Choi) 하보근(Bo-Keun Ha)
• 간행물 정보: 『한국작물학회지』제68권 제4호, 294~303쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

영문 초록

Lipoxygenase gives soybeans their grassy flavor, which can disrupt food processing efficiency. This study aimed to identify soybean genotypes with lipoxygenase deficiency among 1,001 soybean accessions and to develop kompetitive allele specific PCR (KASP) markers that can detect lipoxygenase mutations. Three lipoxygenase isozymes (Lox1, Lox2, and Lox3) were analyzed using a colorimetric assay based on a substrate-enzyme reaction. Among the 1,001 accessions examined, two (IT160160 and IT276392) exhibited a deficiency solely in Lox1, and one (IT269984) lacked both Lox1 and Lox2. IT160160 had a 74-bp deletion in exon 8 of Lox1 (Glyma13g347600), whereas IT276392 displayed a missense mutation involving the change of C to A at position 2,880 of Lox1. Moreover, we successfully developed four KASP markers that specifically target Lox1, Lox2, and Lox3 mutations. To validate the Lox1 KASP markers, we used two F2:3 populations generated through a cross between Daepung 2 (lipoxygenase wild type, maternal parent), IT160160, and IT276392 (null Lox1, paternal parent). The results revealed that the Daepung 2 × IT160160 group followed the expected 3:1 ratio according to Mendel’s law, whereas the Daepung 2 × IT276392 group did not. Furthermore, a comparison between the colorimetric and KASP marker analyses results revealed a high agreement rate of 96%. KASP markers offer a distinct advantage by allowing the distinction of heterozygous types independent of other variables. As a result, we present an opportunity to expedite the lipoxygenase-deficient cultivar development.

목차

MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKNOWLEDGEMENT

REFERENCES

키워드

KASP marker lipoxygenase (Lox) soybean