Expression, purification, and crystallization of human CK2α in complex with the natural flavonoid quercetin

• 영문명: Expression, purification, and crystallization of human CK2α in complex with the natural flavonoid quercetin
• 발행기관: 한국구조생물학회
• 저자명: Anna Cho Danbi Yoon Jiyoung Park Jeong Seok Cha Jiho Yoo
• 간행물 정보: 『Biodesign』Vol 11, No 4, Dec, 74~79쪽, 전체 6쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2023.12.31

영문 초록

Over 300 polypeptide substrates can undergo catalysis by the constitutively active Ser/Thr kinase, human casein kinase 2 alpha (hCK2α). Quercetin, a naturally occurring polyphenolic compound, is well-known for inhibiting hCK2α, thereby regulating cellular signaling pathways. In this study, we crystallized the hCK2α-quercetin complex to elucidate the inhibitory mechanism of quercetin on hCK2α through the complex structure. The hanging drop vapor diffusion method was employed for crystallizing the hCK2α-quercetin complex, and the resulting crystal diffracted to a resolution of 2.11 Å. X-ray diffraction data analysis revealed that the crystal of the hCK2α-quercetin complex belonged to the P43212 space group, with unit cell dimensions of a = b = 127.604 Å, c = 124.314 Å, and α = β = γ = 90.00°. In an initial density map, quercetin was bound well into hCK2α.

목차

INTRODUCTION

RESULTS AND DISCUSSION

MATERIALS AND METHODS

ACKNOWLEDGEMENTS

CONFLICT OF INTEREST

AUTHOR CONTRIBUTIONS

REFERENCES