학술논문
Crystal structure of Bacillus subtilis glyceraldehyde-3-phosphate dehydrogenase GapB
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- 영문명
- Crystal structure of Bacillus subtilis glyceraldehyde-3-phosphate dehydrogenase GapB
- 발행기관
- 한국구조생물학회
- 저자명
- Pawan Dahal Deepak Pathak Eunju Kwon
- 간행물 정보
- 『Biodesign』Vol 11, No 4, Dec, 59~65쪽, 전체 7쪽
- 주제분류
- 자연과학 > 생물학
- 파일형태
- 발행일자
- 2023.12.31
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국문 초록
영문 초록
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) catalyzes the interconversion of glyceraldehyde 3-phosphate and 1,3-diphosphoglycerate during glycolysis and gluconeogenesis. In most organisms, a single GAPDH is responsible for the reaction. However, Bacillus subtilis has two isoforms of this enzyme, namely, GapA and GapB, each of which catalyzes the reaction in the opposite direction. GapA uses NAD+ as a cofactor, whereas GapB prefers NADP+. In this study, we report the crystal structure of GapB at 2.3 Å resolution. X-ray diffraction of GapB crystal was observed up to 2.0 Å resolution, and its structure was determined using molecular replacement. Its overall fold and quaternary structure (a homo-tetramer) were similar to that of the reported GAPDHs. The S-loop was missing because of the absence of the NADP+.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
ACKNOWLEDGEMENTS
CONFLICT OF INTEREST
REFERENCES
키워드
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