Candida auris Aldo-keto reductase AldO: purification, crystallization, and X-ray crystallographic analysis

• 영문명: Candida auris Aldo-keto reductase AldO: purification, crystallization, and X-ray crystallographic analysis
• 발행기관: 한국구조생물학회
• 저자명: Jie Zhang Xue Bai Shanru He Yongbin Xu
• 간행물 정보: 『Biodesign』Vol 11, No 3, Sep, 45~49쪽, 전체 5쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2023.09.30

영문 초록

Candida auris is a new fungus that poses a serious global health threat due to its resistance to multiple antifungal medications, making treatment challenging. Aldo-keto reductase (AKR) represents a versatile superfamily of enzymes that play pivotal roles in the phase I metabolism of various metabolic and detoxification processes. In this study, AKR from C. auris (CaAldO) was successfully expressed and purified using Ni-NTA affinity, Q anion exchange, and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 1.95 Å. The crystal belonged to the orthorhombic space group P212121, with unit-cell parameters of a = 71.65, b = 91.15, and c = 227.66 Å. The Matthews coefficient and solvent content were estimated to be 2.32 Å3 Da-1 and 47.00%, respectively, assuming the asymmetric unit contained four recombinant protein molecules.

목차

INTRODUCTION

RESULTS AND DISCUSSION

METHODS

ACKNOWLEDGEMENTS

CONFLICT OF INTEREST

REFERENCES