Cryo-electron tomography: a triumphant breakthrough in structural biology

• 영문명: Cryo-electron tomography: a triumphant breakthrough in structural biology
• 발행기관: 한국구조생물학회
• 저자명: Han-ul Kim Mi Young An Hyun Suk Jung
• 간행물 정보: 『Biodesign』Vol 11, No 3, Sep, 33~38쪽, 전체 6쪽
• 주제분류: 자연과학 > 생물학
• 파일형태: PDF
• 발행일자: 2023.09.30

영문 초록

Cryo-electron microscopy (Cryo-EM) has been offering impressive insights into the structures of macromolecules in the field of structural biology as a result of recent advancements in hardware and software technologies. This breakthrough has greatly aided our understanding of the cellular mechanisms of proteins and has triggered the dawn of an unprecedented golden age in structural biology. However, the technique still has inherent limitations owing to the dynamic tendency and tangled interactions of biomolecules. Attempts to overcome these limitations have led to the emergence of cryo-electron tomography (Cryo-ET) as a powerful technique in the field of structural biology, allowing for the three-dimensional visualization of the native conformation of biological specimens. This promising electron microscopy method has revolutionized our grasp of complex structures and their dynamic interactions, thereby providing unprecedented insights into the organization and function of macromolecular complexes within their native cellular environments. In this paper, we review state-of-the-art cryo-ET workflows, provide examples of biological applications, and discuss the fundamental necessity of boosting the potential applications of cryo-ET.

목차

INTRODUCTION TO CRYO-EM TECHNOLOGY

GENERAL CONCEPTS OF TEM TO VISUALIZE BIOLOGICAL MOLECULES

PREPARATIVE WAY OF IMAGE ACQUISITION TO OBTAIN ELECTRON TOMOGRAM

TECHNOLOGICAL DEVELOPMENTS OF DED ACHIEVABLE HIGH-RESOLUTION CRYO-ET

STRUCTURAL DETERMINATION OF MACROMOLECULES BY USING CRYO-ET

HIGH-RESOLUTION CRYO-ET APPLICABLE TO LIFE SCIENCES

ACKNOWLEDGEMENTS

CONFLICT OF INTEREST

AUTHOR CONTRIBUTIONS

REFERENCES