Development of Cleaved Amplified Polymorphic Sequence Markers for Classifying Ginger (Zingiber officinale) Cultivars Using Reference Sequencing

• 영문명: Development of Cleaved Amplified Polymorphic Sequence Markers for Classifying Ginger (Zingiber officinale) Cultivars Using Reference Sequencing
• 발행기관: 한국육종학회
• 저자명: Ji-Nam Kang Gyeong-Hui Lee Jin Yu Mi-Hwa Choi Simyung Lee
• 간행물 정보: 『Plant breeding and biotechnology』Vol.11 No.2, 130~140쪽, 전체 11쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.06.30

영문 초록

Ginger (Zingiber officinale) is grown worldwide in subtropical and tropical regions and primarily used as a spice and medicinal plant. Despite the economic importance of ginger, research on its molecular aspects is limited. Moreover, although ginger is mainly cultivated through vegetative propagation owing to poor flowering and infertility, few molecular markers have been identified to distinguish cultivars. In this research, we developed five Cleaved Amplified Polymorphic Sequence (CAPS) markers that can distinguish between the “Bongdong” ginger (Bg) cultivar, indigenous to South Korea, and the Chinese imported ginger (Cg) cultivar through reference sequencing based on the recently reported complete genome information of ginger. Furthermore, the integrated application of the five CAPS markers allow us to distinguish between Bg, Cg, and Indonesian ginger. Among them, the ClaI-based CAPS marker was identified as specific to Bg cultivars. Therefore, TaqMan real-time PCR based on ClaI-based CAPS can be widely used to distinguish between Bg and Cg cultivars. This study is the first to report the development of genome-based single-nucleotide polymorphism markers in ginger and therefore provides important information for the breeding and conservation of ginger.

목차

INTRODUCTION

METERIALS AND METHODS

RESULTS

DISCUSSION

AUTHOR CONTRIBUTIONS

ACKNOWLEDGEMENTS

DATA AVAILABILITY STATEMENT

FUNDING

REFERENCES

키워드

Ginger CAPS marker TaqMan real-time PCR