학술논문
In vitro antioxidant and free radical scavenging activities of stem extract of Euphorbia trigona Miller
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- 영문명
- 발행기관
- 셀메드 세포교정의약학회
- 저자명
- Raj Kumar Salar Pooja Sharma Sukhvinder Singh Purewal
- 간행물 정보
- 『셀메드』Volume 5 Issue 2, 1~6쪽, 전체 6쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 2015.05.31
무료
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국문 초록
영문 초록
Antioxidative and free radical scavenging properties of different stem extracts of Euphorbia trigona were evaluated and correlated with its total phenolic content. Aqueous, acetone and methanolic extracts of shade dried stem were obtained and were concentrated in vacuo. The antioxidant and free radical scavenging activities of stem extracts was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, deoxyribose degradation assay and Fe2+chelating assay. Total phenolic contents (TPC) were evaluated using Folin-Ciocalteu reagent. The results confirmed that the plant is a rich source of polyphenolic compounds which are invariably higher compared to other herbs.
All extracts showed TPC in the range of 146.6 – 168.6 mg/g gallic acid equivalents at 300 μg/ml of extract.
Among the three extracts ME showed highest scavenging activity as evidenced by maximum scavenging of DPPH (83.2%), OH• radicals (94.81%), Fe2+chelating activity (88.59%) and a high reducing power 0.623 at 300 μg/ml. Our results demonstrate that Euphorbia trigona, an unexplored xerophytic plant could be potential source of natural antioxidants and phytotherapeutic agents. The plant possess invariably high amount of polyphenolic compounds with a broad spectrum of antioxidant properties and could be further used for food, feed and pharmaceutical applications.
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