Effects of the Fraction of Sambucus Williamsii, NNMBS 246, on Osteoblastic Differentiation

• 발행기관: 셀메드 세포교정의약학회
• 저자명: Soon-Il Kang Jaesuh Park Il-Keun Kwon Eun-Cheol Kim
• 간행물 정보: 『셀메드』Volume 8 Issue 3, 1~8쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2018.08.31

영문 초록

In the field of osteoporosis, there has been growing interest in anabolic agents that enhance bone formation. The purpose of this study was to examine the effects of NNMBS 246 osteoblastic differentiation with associated signaling pathways. NNMBS 246 markedly increased alkaline phosphatase (ALP) activity and calcium nodule formation. Stimulation with NNMBS 246 not only increased the differentiation markers (ALP, OPN, OCN) level and transcription markers (RUNX2, Osterix) mRNA expression but also upregulated the ECM molecules and OPG mRNA expression. Treatments of NNMBS 246 downregulated MMPs (MMP-1, MMP-2, MMP-9), but RANKL mRNA expression. Furthermore, NNMBS 246 activated osteoblastic differentiation markers and formed calcium nodules in human periodontal ligament cells (hPDLCs) and cementoblast cells. NNMBS 246 induced phosphorylation of MAPKs, Akt, nuclear p65 and IkB-α. BMP-2/Smad and β-catenin signaling pathways were activated by NNMBS 246. Sirtinol (SIRT1 inhibitor) inhibited NNMBS 246-induced osteoblastic differentiation markers mRNA expression. These results suggested that NNMBS 246 has the potential to enhance osteoblastogenesis probably through the activation of BMP/Smad and β-catenin signal pathways, and SIRT1 plays as critical mediator in bone anabolic effect of NNMBS 246.

키워드

NNMBS 246 Osteoblast Differentiation BMP Pathway SIRT1 Pathway