개인식별을 위한 STR 마커의 개발

• 영문명: The Development of STR Marker for Human Identification
• 발행기관: 한국의료정보교육협회
• 저자명: 신창호(Chang-Ho Shin)
• 간행물 정보: 『보건의료생명과학논문지』Vol.1 No.1, 101~108쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2013.12.30

영문 초록

The development of polymerase chain reaction(PCR) method with short tandem repeat (STR) markers is very useful tool for human identification in forensic cases. PCR method is lots advantages than the Amp-FLP method. This method can be performed by small amount DNA templates, quickly time and low cost. STR marker still concentrates on many forensic scientists since reporting PCR as a human identification method in 1885. In order to optimal condition of PCR amplification with GATA67G11 locus. There is tested condition for various annealing temperature and time, template DNA amount, wide range cycle numbers and primer concentration for optimal amplification. In this study, sensitive PCR was achieved by optimizing parameters such as 0.4 pmole primers, and 10ng template DNA. Optimal amplifying condition of GATA67G11 locus was 61℃ for 20 second. Therefore, it would take a optimal condition for 95℃, 10 sec., annealing for 61℃, 20sec., extension for 72℃, 10sec., repeating 30 cycles before final extension 72℃, 10 min. The sequencing data of small single allele were showed two repetitive units, three TAGG repeating numbers and eight ATAG repeating numbers, 11 repeating numbers totally. In conclusion, optimal PCR condition in GATA67G11 locus was studied to apply the individual human identification with DNA specimens. There resulted in success in all specimens and confirmed many methods for individual identification with GATA67G11 locus.

목차

Ⅰ. 서 론

Ⅱ. 연구 재료 및 방법

Ⅲ. 연구 결과

Ⅳ. 고 찰

참고문헌

키워드

PCR STR marker GATA67G11 human identification forensic