Identification of the Housekeeping Genes Using Cross Experiments via in silico Analysis

• 발행기관: 한국작물학회
• 저자명: Won Cheol Yim Changwon Keum Saehwan Kim 장철성(Jang, Cheolseong) 이병무(Byung-Moo Lee)
• 간행물 정보: 『한국작물학회지』제55권 제4호, 371~378쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2010.12.30

영문 초록

For sensitive and accurate gene expression analysis, normalization of gene expression data against housekeeping genes is required. There are conventional housekeeping gene (e.g. ACT) that primarily function as an internal control of transcription. In this study, we performed an in silico analysis of 278 rice gene expression samples (GSM) in order to identify the gene that is most consistently expressed. Based on this analysis, we identified novel candidate housekeeping genes that displayed improved stability among the cross experimental conditions. Furthermore four of the most conventional housekeeping genes were included in our 30 other housekeeping genes among the most stable genes. Therefore, these 30 genes can be used to normalize transcription results in gene expression studies on rice at a broad range of experimental conditions.

목차

MATERIALS AND METHODS

RESULTS & DISCUSSION

ACKNOWLEDGEMENT

REFERENCES

키워드

housekeeping genes normalization GEO microarray