콩 함유 사포닌의 종류 및 함량 분석

• 영문명: HPLC/MS/MS Method for Determination of Soyasaponins in the Soybean Varieties
• 발행기관: 한국작물학회
• 저자명: 한상준(Sang-Jun Han)
• 간행물 정보: 『한국작물학회지』제56권 제3호, 244~249쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.09.30

영문 초록

A sensitive and rapid high-performance liquid chromatographic-tandem mass spectrometric (HPLC/MS/MS) assay was developed for the determination of soyasaponins in soybean. Among soyasaponins, soyasaponin I was isolated and characterized from methanol extracts of soybean as analytical standards and the development of a new analytical procedure for quantification of its content in various cultivars. The structures of these compound was elucidated by 1H, 13C NMR experiments and by mass spectrometric analysis. Aqueous ethanol extracts of soybean samples were injected on an Agilent XDB-C18 column (4.6 mm × 50 mm, 1.8 μm) with a mobile phase consisting of 10 mM ammonium acetate-acetonitrile, a flow rate of 0.3 mL/min and a total run time of 8 min. Detection was performed by mass spectrometer bin the multiple reaction monitoring (MRM) mode with negative electrospray ionization (ESI) m/z at 941 ⟶ 615 for soyasaponin I. In the 9 soybean samples, contents of soyasaponin I ranged from 205 to 726 mg/kg, and correlated negatively with seed size.

목차

재료 및 방법

결과 및 고찰

적요

사사

인용문헌

키워드

soybean soyasaponin HPLC/MS/MS QQQ