Regeneration Potential of Immature Embryos during Seed Development in Spring and Winter Wheat Genotypes

• 발행기관: 한국작물학회
• 저자명: Kyung-Hee Kim Ji-Suk Park Byung-Moo Lee
• 간행물 정보: 『한국작물학회지』제56권 제3호, 279~283쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.09.30

영문 초록

The immature embryos during seed development were examined to predict the suitable embryos for an efficient regeneration system. Five spring wheat genotypes and five winter wheat genotypes were tested using immature embryos as explants. Spring wheat genotypes showed much higher levels of plant regeneration than those of winter wheat genotypes. The highest frequencies of embryogenesis and regeneration were obtained when embryos at 13-14 days after anthesis (DAA) were used as explant and decreased using embryos at 21-22 DAA during seed development. Significant differences were also found for callus induction and regeneration as affected by immature embryo size. The regeneration efficiency was drastically decreased in spring and winter wheat genotypes when embryos larger than 2.0 mm of length were used. The optimum developmental stage and embryo length for regeneration efficiency were at 13-14 DAA and 1.0-1.5 mm, respectively. The selection of suitable embryos for the high frequencies of embryo-genesis and regeneration leads us to efficient genetic improvement of wheat.

목차

MATERIAL AND METHODS

RESULTS AND DISCUSSION

ACKNOWLEDGEMENT

REFERENCES

키워드

wheat regeneration seed development immature embryo