수수종자의 펩타이드 분석을 위한 SELDI-TOF MS 최적화 연구

• 영문명: Optimization of SELDI-TOF MS for Peptide Profiling of Sorghum Seed
• 발행기관: 한국작물학회
• 저자명: 박세준(Sei Joon Park) 박준영(June Young Park) 이용호(Yong Ho Lee) 황수민(Su Min Hwang) 김아람(A Ram Kim) 고지연(Jee-Yeon Ko) 김태완(Tae Wan Kim)
• 간행물 정보: 『한국작물학회지』제58권 제1호, 50~56쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.03.30

영문 초록

For accurate analysis of low molecular peptides using SELDI-TOF MS (surface enhanced laser desorption/ ionization time of flight mass spectrometry), the optimized analytical conditions should be established for a specific biological sample. This study was conducted to optimize SELDI-TOF MS analytical conditions for profiling low molecular peptide below 10 kDa presented in sorghum seeds. Analytical conditions were as follows; (1) protein chips: CM10 (weak cation exchanger) and Q10 (strong anion exchanger), (2) dilution factors of binding buffer: 1/2, 1/5, 1/10, 1/20, 1/50, 1/100, and 1/200, (3) the stringency of Q10 binding buffer: 10 mM and 100 mM, and (4) protein extrac-tion buffers: sodium borate, sodium borate + acetone, phenol, and TCA buffers. Optimum dilution factors were selected as 1/20 and 1/50 in both protein chips, CM10 and Q10. Low stringency of Q10 binding buffer (10mM) detected more peptide peaks than high stringency (100 mM). Selected protein extraction buffers of sorghum seed for SELDI-TOF MS analysis was the sodium borate buffer in the range of 2~10 kDa, while the phenol buffer was more suitable in the range of 10~20 kDa.

목차

재료 및 방법

결과 및 고찰

적요

사사

인용문헌

키워드

sorghum peptide SELDI-TOF MS optimization