HepG2 세포에서 용매에 의한 차별적인 사람 싸이토크롬 P450 2E1활성 변화

• 영문명: Differential Role of Solvents on Human Cytochrome P450 2El Activity in Intact HepG2 Cells
• 발행기관: 한국환경보건학회
• 저자명: 최달웅(Dal Woong Choi)
• 간행물 정보: 『1. 한국환경보건학회지』제29권 제3호, 9~15쪽, 전체 7쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2003.09.30

영문 초록

The modification of CYP2El activity is a matter of considerable interest because of its role in the metabolic activation of a variety of environmental toxicants. In the present study, the time-course of changes in human CYP2El activities was determined following treatment with solvents (acetone, dimethylsulphoxide or pyridine) using intact HepG2 cells transfected by human CYP2El. Hydroxylation of chlorzoxazone was used for the measurement of CYP2El activity. CYP2E1 protein level was increased upon cultivation of cells in the presence of the solvents for 24 hr. Determination of CYP2El activities after 24 ht cultivation with the solvents demonstrated that acetone or dimethylsulphoxide increased, whereas pyridine inhibited the activities. This differential effect of the solvents on CYP2El activities persisted to subsequent 24 ht. Competitive inhibition study suggested that pyridine has stronger binding affinity to CYP2E1 than acetone or dimethylsulphoxide. These results demonstrate that different binding affinity of the solvents to CYP2El plays important role in determining real CYP2El activity in intact cells after exposure to the solvents. Present study would be helpful in precise understanding of human CYP2El-mediated toxicity.

목차

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 결과 및 고찰

Ⅳ. 결 론

감사의 글

참고문헌

키워드

Cytochrome P450 2E1 (CYP2E1) Activity Acetone Dimethylsulphoxide Pyridine