확률론적 모의실험을 이용한 공기청정기의 실내공기중 PM10과NO2 제거효율에 관한 연구

• 영문명: Efficiency of Removal for PM10 and NO2 by Air Cleaner inResidential Indoor Enviromental with Monte-Carlo Simulation
• 발행기관: 한국환경보건학회
• 저자명: 이철민(Cheol-Min Lee) 김윤신(Yoon-Shin Kim) 이태형(Tae-Hyung Lee) 김종철(Jong-Cheol Kim) 김중호(Jung-Ho Kim)
• 간행물 정보: 『1. 한국환경보건학회지』제30권 제3호, 221~229쪽, 전체 9쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2004.09.30

영문 초록

The adverse health effects of a number of environment pollutions are related to the formation of free radicals. Induction of antioxidant defensive system in the response to an oxidative attack is an essential element of the cell to survive. CYP2E1 is easily induced by organic solvents and induces continuous formation of reactive oxygen species (ROS). g-Glutamyltranspeptidase (gGT) plays an important role in glutathione metabolism and xenobiotic detoxification. To evaluate the characteristic of oxidative stress which induces GGT expression and to understand human antioxidant defensive response against oxidative stress induced by CYP2E1, we studied regulation of gGT enzyme expression in response to various oxidative stresses in human HepG2 cells. The gGT activity was not modified after exposure of acute oxidative stress inducing agents (ferric nitrilotriacetate, cumene hydroperoxide, ADP-Fe, O-tetradecanoylphorbol-13-acetate, tumor necrosis factor-alpha). To induce continuous exposure of cells to ROS, HepG2 cells were transfected by human CYP2E1 gene transiently. The CYP2E1 activity was verified with chlorzoxazone hydroxylation. Transfection of CYP2E1 showed continuous 60% increase in intracellular ROS and 240 % increase in microsomal ROS. CYP2E1 overexpressing cells showed increased gGT activity (2.5-fold). The observed enhancement of gGT activity correlated with a significant increase of gGT mRNA (2.1-fold). Treatment with antioxidant strongly prevented the increase in gGT activity. The CYP2E1 overexpression did not modify toxicity index and increased glutathione levels. These results show that continuous exposure of cells to ROS produced by CYP2E1 up-regulates gGT; This may be one of the adaptive antioxidant responses of cells to oxidative insult. Present study also suggests that the induction of gGT could be used as a marker of oxidative stress induced by exposure to organic solvents.

목차

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 결과 및 고찰

Ⅳ. 고찰

Ⅴ. 결론

키워드

g-Glutamyltranspeptidase Reactive oxygen species mRNA organic solvent