ICR mouse의 피부와 간에서 자외선 B파에 의한 Heme Oxygenase-1의 유도

• 영문명: Heme Oxygenase-1 (HO-1) Induction by UVB (290-320nm) Radiation in ICR Mice
• 발행기관: 한국환경보건학회
• 저자명: Choi, Wook-Hee Kim, Tae-Hwan Ahn, Ryoung-Me
• 간행물 정보: 『1. 한국환경보건학회지』제34권 제1호, 49~54쪽, 전체 6쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2008.02.28

영문 초록

The induction of heme oxygenase-1(HO-1) by UV radiation provides a protective defence against oxidative stress, and has been well demonstrated in skin irradiated with UVA, but not UVB. In this study, we show that the induction of cutaneous HO-l can be attributed to UVB radiation. The expression of HO-1 mRNA was assessed in vivo by reverse transcription-polymerase chain reaction (RT-PCR) analysis, and HO-1 enzyme activity was measured in microsomal preparation from irradiated mice. The mRNA level of HO-1 increases in liver and skin from 1d to 3d after UVB (3KJ/m2) exposure. The results of gene expression were same pattern of HO-1 enzyme activity in skin, but not in liver. HO-1 mRNA in liver resulted in a progressive increase to 4d after UVB exposure, but HO-1 activity in liver increased to 2d. This finding indicates that UVB radiation is an important inducer of HO-1 and increases in HO activity may protect tissue directly or indirectly from oxidative stress.

키워드

UVB radiation Heme Oxygenase-1 mouse skin & liver