Expression Analysis of ESTs Derived from the Leaf of Chunpoong (Panax ginseng C,A. Meyer)

• 영문명: Expression Analysis of ESTs Derived from the Leaf of Chunpoong (Panax ginseng C,A. Meyer)
• 발행기관: 한국자원식물학회
• 저자명: In, Jun-Gyo Lee, Bum-Soo Yang, Deok-Chun
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2003년도 춘계 학술발표대회, 122~122쪽, 전체 1쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2003.04.30

영문 초록

In order to study gene expression transcribted during the embryo development, we constructed a cDNA library of embryogenic callus induced from cotylendon of Korean ginseng and generated expressed sequence tags (ESTs) of 3,359 clones randomly selected. The ESTs could be clustered into 1,910 (59.1%) non-redundant groups. Similarity search of the non-redundant ESTs against public non-redundant databases of both protein and DNA indicated that 2,217 groups show similarity to genes of known function. These ESTs clones were divided into eighteen categories depending upon gene function. Most abundant transcripts were ribosomal protein small subunit 28kDa(40), tumor-related protein(35), metallothionein (31), small heat-shock protein class 18.6K(24), and cyclophilin(20). There are no useful informations of gene expression during the embryo development in Korean ginseng. These results could help to understand the embryo development in Korean ginseng.