호남지역 재래종 들깨의 작물학적 형질에 관한 연구

• 영문명: Studies on the Agronomic Characters of Local Perilla Collected in the Honam Part of Korea
• 발행기관: 한국자원식물학회
• 저자명: 남상영 김인재 김민자 이철희 김태수
• 간행물 정보: 『한국자원식물학회 학술심포지엄』2002년도 제9차 국제심포지움 및 추계정기학술발표회, 21~22쪽, 전체 2쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2002.11.30

영문 초록

We established hairy root cultures of F. esculentum transformed with A. rhizogenes for in vitro rutin production. Additionally, we describe the effects of different media and plant growth regulators on growth and rutin biosynthesis in buckwheat hairy root cultures. Excised leaves of P. tinctorium from 10-day-old seedlings were used as the explant material for co-cultivation with A. rhizogenes 15834. The hairy culture of Fagopyrum esculentum Moench. was established by infecting leaf explants with Agrobacterium rhizogenes 15834. About four to five weeks after co-cultivation with A. rhizogenes, 10 hairy roots were excised from the necrotic explant tissues. After repeated transfer to fresh medium for three months, ten clones were transferred to MS liquid culture medium. The growth and rutin production of each clone differently response to the MS liquid medium. Among these clones, H8, which had exhibited good growth rate and one of the highest rutin productivity, was selected for the following experimment.(중략)