유색칼라 기내 미세번식에 미치는 식물생장조절물질의 영향

• 영문명: Effect of Plant Growth Regulators on in vitro Micropropagation of Colored Calla Lily(Zantedeschia spp.)
• 발행기관: 한국자원식물학회
• 저자명: 이영순(Young Soon Lee) 고정애(Jeong Ae Ko)
• 간행물 정보: 『한국자원식물학회지』제18권 제1호, 154~160쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2005.02.28

영문 초록

To establish rapid micropropagation through organogenesis from apices-derived callus or direct adventitious shoot of three calla lily cultivars(Zantedeschia spp, cv. Sunlight, cv. Chiante, cv. Pink Persuation) were cultured on Murashige and Skoog medium supplemented with different plant growth regulators. The formation rate of callus, organogenesis and in viかo tuber production among the three cultivars were tested. Callus was obtained from cvs. Sunlight, Chiante and Pink Persuasion; the best cultivar was Sunlight. Sunlight induced 53.3% callus and Chiante had the highest rate of 56.7% direct shoot regeneration on medium with 2.0 mg/L BA. Regeneration frequencies ranged from 20 to 70% on medium with 2.0-3.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoots were obtained on medium containing 3.0 mg/L BA in three cultivars. Cytokinins induced multiple shoot formation; 1.0 mg/L of 2ip, 5.0 mg/L of BA, and 1.0 m/L of BA induced 16, 14 and 12 multiple shoots in cvs. Sunlight, Chiante and Pink Persuasion, respectivly. 1.0 mg/L of IAA enhanced root growth in cvs. Sunlight and Chiante while cv. Pink Persuasion exhibited enhanced root growth at 2.0 mg/L of IBA. NAA, however, induced no change in root growth. The addition of 90 g/L sucrose enhanced in vitro tuber formation and following tuber expansion in cv. Sunlight, while 70 g/L of sucrose was effective in cvs. Chiante and Pink Persuasion.

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키워드

adventitious shoot formation growth regulators in vitro culture sucrose tuberization