Agrobacterium spp. 에 의하여 형질전환된 감자조직의 생장특성

• 영문명: Growth Characteristics of Transgenic Potato Using Wild-type Agrobacterium spp.
• 발행기관: 한국자원식물학회
• 저자명: 양덕춘
• 간행물 정보: 『한국자원식물학회지』제11권 제2호, 176~187쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1998.06.30

영문 초록

This study was carried out to obtain the information for growth characteristics of crown gall tumor and hairy root transformed by Agrobacterium spp,. on the media with phytohormones, casein hydrolysate and activated charoal. Crown gall tumors and hairly roots were formed respectively on potato tuber discs infected by tumerfaciens A ch 5 and A.rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. PCR analysis of Rol C and Vir C gene fragments confirmed that crown gall root was prompted on the medium containing 2,4-D 2mg/l with casein hydrolysate lg/l. The survival ration of crown gall tumor callus derived from potato increased on medium containing the activated charcoal 0.5∼0.2mg/l because of the prevention, on the other hand, hairly roots were necrosis on the same medium. Callus derived from hairly root were excellently grown for a short time by suspension culture on liquid medium containing 2.4-d 2mg/L and casein hydrolysate lg/l.

키워드

activated charcoal casein hydrolysate crown gall tumor hairy root Rol C. Vir C