흰나리 인편으로부터 PR-Proteinase의 유도 및 특성 규명

• 영문명: Induction by Salicylic Acid and Characterization of PR-Proteinases from Bulbs of Lilium formosanum Wallace
• 발행기관: 한국자원식물학회
• 저자명: 오순자
• 간행물 정보: 『한국자원식물학회지』제11권 제2호, 195~201쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1998.06.30

영문 초록

Some morphological character were surveyed and PR-proteinases were induced and characterized from Lilium formosanum Wallace endeimc to Cheju island . Its flower characters were similar to those of white trumpet lilies(Lilium longiflorum Thunb) although its flowering period was later than that of white trumpet lilies and it hadd a wide range of variation among individuals. Six PR-proteinases(II-2, III-1, III-2, IV-1, IV-2 and V) were induced from bulbs by 2.5mM salicylic acid and almost excreted into the intercellular spaces. These PR-proteinases were strongly activated by Ca 2+, , whereas they were strongly inhibited by Cu2+ Co2+ and Fe2+ . Three PR proteinases(II-2, IV-1 and IV-2) were strongly inhibited by 1, 10 -phenanthroline, indicating that these enzymes are metallo-proteinases. Three PR-proteinases(III-1, III-2 and V) had a high sensitivity to PMSF and required β-mercaptoethanol for their activities. These results indicate that these proteinases are cysteine proteinases.

키워드

PR-proteinases salicylic acid metallo-proteinases cysteine proteinases Lilium formosanum