The Protective and Inhibitory Effect of Antioxidants Found in Broussonetia kazinoki Siebold against Oxidative DNA Damage

• 발행기관: 한국자원식물학회
• 저자명: Tae-Won Jang Ji-Soo Choi Hoi-Ki Kim Eun-Ja Lee Ki-Beom Lee Tae-Hyung Kwon Do-Wan Kim Jeong-Jwa Ahn Jae-Ho Park
• 간행물 정보: 『한국자원식물학회지』제32권 제6호, 714~722쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Oxidative DNA damage negatively affects humans and the research is currently ongoing to find ways to reduce oxidative stress. Oxidative stress has been identified as a key factor in triggering various diseases. Thus, its alleviation is important for human health. Broussonetia kazinoki (B. kazinoki) has been used in traditional Korean medicine as a dermatological therapy to treat burns, pruritus, and acne. B. kazinoki is generally segregated into peeled root (PR), root bark (RB), peeled stem (PS), and stem bark (SB). To assess these components for their antioxidant activity and protection against DNA damage, their ethyl acetate fractions were examined by 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2 -Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assay. As a result of confirming the expression of factors involved in attenuating DNA damage, the protective effect of SB on oxidative stress suppressed the expression of p-p53 and γ-H2AX. Additionally, the levels of p53 and H2AX mRNA were significantly downregulated. In conclusion, these results indicated that the SB component of B. kazinoki had the potential to be used as an effective natural antioxidant compared to the other parts of the plant.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgments

References

키워드

Broussonetia kazinoki Siebold DNA Damage p53 γ-H2AX