세균을 이용한 CA약침의 복귀돌연변이 시험

• 영문명: Bacterial Reverse Mutation Test of CA-YAKCHIM
• 발행기관: 임상약침학회
• 저자명: 안덕근(Duck-Gun An) 정철(Chul Jung)
• 간행물 정보: 『임상약침학회학회지』대한면역약침학회지 제3권 제1호, 39~47쪽, 전체 9쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2014.06.30

영문 초록

Objectives : The purpose of this study was to determine the mutagenic potential of CA-YAKCHIM using histidine requiring Salmonella typhimurium (TA98, TA100, TA1535, and TA1537) and tryptophan requiring Escherichia coli(WP2uvrA(pKM101)) strains in the absence and presence of metabolic activation. Methods : In order to determine the dose levels of the main study, the dose range finding study was performed first. The high dose was selected at 5,000 μg/plate and it was sequentially diluted by the geometric ratio of 4 to 1,250, 313, 78.1, and 19.5 μg/plate. As a result, the growth inhibition by the CA-YAKCHIM was not evident at any dose levels in the absence and presence of metabolic activation. Therefore, the dose levels of the main study were selected as 313, 625, 1,250, 2,500, and 5,000 μg/plate. And the negative (acetone) and positive (sodium azide, 2-Nitrofluorene, 9-Aminoacridine, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide, and 2-Aminoanthracene) control groups were used. Results : The mean number of revertant colonies was less than twice when compared to the negative control values at all dose levels of the CA-YAKCHIM in the presence and absence of metabolic activation, without dose-related increase. In the positive control group, the number of revertant colonies was markedly increased when compared to that of the negative control group. Conclusions : In this bacterial reverse mutation test, CA-YAKCHIM did not exhibit any indications of mutagenic potential.

목차

Ⅰ. 서 론

Ⅱ. 재료 및 방법

Ⅲ. 연구결과

Ⅳ. 고찰 및 결론

참고문헌

키워드

CA-YAKCHIM IMMUNO-YAKCHIM Toxicity Bacterial reverse mutation test pharmacopuncture Nam Sang Cheon