해수로부터 Urease 활성 Photobacterium sp. Strain HA-2 분리

• 영문명: Isolation of Urease-Positive Photobacterium sp. Strain HA-2 from Sea Water
• 발행기관: 한국산업기술융합학회(구. 산업기술교육훈련학회)
• 저자명: 고하영(Ha-Young Koh)
• 간행물 정보: 『산업기술연구논문지』산업기술연구논문지 제25권 1호, 23~30쪽, 전체 8쪽
• 주제분류: 공학 > 공학일반
• 파일형태: PDF
• 발행일자: 2020.03.30

영문 초록

Urease catalyzes the hydrolysis of urea into ammonia and carbamate. The latter spontaneously decomposes into another ammonia molecule and carbonic acid. We isolated a urease-positive bacterium from seawater on the western coast of Gomso bay in Korea. Using biochemical, morphological, and 16S rRNA gene analyses, we identified the bacterium as a Photobacterium sp. and named it Photobacterium sp. strain HA-2. We observed that the Photobacterium sp. strain HA-2 showed a 2.0-fold difference in urease activity, when cultured in an LB medium containing 3% NaCl with or without 0.1% urea (0.1620 vs. 0.0820 μmol NH3min/mgprotein) In this study, we also cloned and sequenced a ca. 15-kb DNA fragment from the chromosome of Photobacterium sp. strain HA-2, containing the urease gene clusters. We found ten open reading frames (ORFs) and the GC-content (40.9%) showed almost a perfect resemblance to the total genome of Photobacterium damselae subsp. damselae (40%). The urease gene cluster consisted of seven genes, and its genetic organization and sequences resembled those found in Vibrio parahaemolyticus and Vibrio fischeri.

목차

Ⅰ. 서 론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험결과 및 고찰

참고문헌

키워드

포토박테리움 우레아제 우레C유전자 클로닝 오픈 리딩 프레임 Potobacterium sp. Urease ureC Gne Cloning ORFs