국가 바이오모니터링 프로그램을 위한 소변 중 금속류 동시분석법 개발 및 검증

• 영문명: Development and Validation of the Simultaneous Analytical Method of Urinary Metals and Metalloids for the National Biomonitoring Programs
• 발행기관: 한국환경보건학회
• 저자명: 조용민(Yong Min Cho) 양민호(Minho Yang) 임호섭(Hosub Im) 차상원(Sangwon Cha) 이재익(Jaeick Lee) 김기훈(Ki Hun Kim) 한상범(Sang Beom Han)
• 간행물 정보: 『1. 한국환경보건학회지』제45권 제6호, 594~604쪽, 전체 11쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2019.12.30

영문 초록

Objectives: This study developed and validated an analysis method of urinary metals and metalloids that can be applied inductively with coupled plasma mass spectrometry (ICP-MS). Methods: 0.3 mL of urine was used to analyze 25 metal and metalloid compounds using ICP-MS. The validation of the analytical method included linearity, accuracy, precision, and the calculation of detection limits. In addition, a comparison test was performed with the graphite furnace atomic absorption spectrometry (GFAAS) method, which is the current standard method, with urine samples of 66 healthy subjects. Results: The linearities (R2) of calibration curves of all 25 compounds were ≥ 0.999. Of the 25 compounds, the intra-day and inter-day accuracy% of 17 and 20 met ≤15%, respectively. In addition, fifteen compounds showed ≤15% recovery% for certificated reference materials. Intraclass correlation coefficients of the comparison between the current methods and new methods in this study were 0.952 (p-value<0.001) and 0.911 (pvalue<0.001) for urinary cadmium and mercury, respectively. Conclusion: This study proposes an efficient simultaneous methodology that can analyze multi elements in smaller sample amounts. More reproduction experiments are needed in the future.

목차

I. 서 론

II. 재료 및 방법

III. 결 과

IV. 고 찰

V. 결 론

키워드

Urinary metals ICP-MS simultaneous analysis biomonitoring