Brassica campestris의 형질전환에 대한 Sodium Hypochlorite의 효과

• 영문명: Effect of Sodium Hypochlorite on Brassica campestris ssp. Transformation via Agroinfiltration
• 발행기관: 한국육종학회
• 저자명: 박희성(Hee Sung Park) 신동일(Dong Il Shin)
• 간행물 정보: 『한국육종학회지』Vol.37 No.5, 300~303쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2005.10.30

영문 초록

Brassica campestris ssp. has been cultivated as oil and vegetable crop. However, it belongs to one of the dicot plant species recalcitrant to Agrobacterium infection for plant transformation and regeneration. We demonstrate that Agrobacterium-mediated transformation via vacuum infiltration poorly works for B. campestris var. narinosa seedlings in histochemical detection of GUS reporter gene expression. Either pre-germinated or germinating seeds were not susceptible to Agrobacterium infection. But, GUS gene expression was successfully achieved when 0.4% sodium hypochlorite solution, as an antimicrobial reagent, was treated for 2 min to two-days-old germinating seeds prior to agroinfiltration. Transformation efficiency was quantitated by using ELISA with another set of transgenic seedlings.

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Abstract

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材料 및 方法

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引用文獻

키워드

Brassica campestris ssp. GUS agroinfiltration sodium hypochlorite ELISA