벼 Ds 삽입변이 계통의 특성변이 및 분자생물학적 특성

• 영문명: Trait Variation and Molecular Characterization of Ds insertional rice lines
• 발행기관: 한국육종학회
• 저자명: 안병옥(Byung-Ohg Ahn) 강경호(Kyung-Ho Kang) 은무영(Moo-Yong Eun) 전용희(Yong-Hee Jeon) 윤도원(Doh-Won Yun) 지현소(Hyeon-So Ji) 박성한(Sung-Han Park) 남민희(Min-Hee Nam) 서석철(Seok-Cheol Suh) 이명철(Myung-Chul Lee)
• 간행물 정보: 『한국육종학회지』Vol.40 No.1, 39~47쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.03.30

영문 초록

The 1,874 rice lines were selected from 3,000 Ds insertional mutant pool by Basta herbicide treatment and were surveyed for trait variation and molecular characteristics of genes knocked out by Ds insertion. Compared with “Donjin”, an original japonica cultivar used for transformation, Ds insertion mutant pool showed large variation in major agronomic traits including tiller, panicle, and heading etc. Southern blot analysis demonstrated that these lines on the average had two Ds copies in Donjin genome, resulting in 38.4% of one copy, 32.5% of two copies, 16.7% of three copies, and 11.3% of over four copies. GUS analysis showed that 3.9% of lines (73/1,860) had tissue-specific expression in leaves, nodal parts, floral organs such as stigma and pollen, and roots. Data set obtained from agricultural trait variation and molecular characteristics for individual Ds insertional lines would provide researchers with more information for understanding the function of unknown rice genes controlling economically important traits.

목차

서 언

재료 및 방법

결과 및 고찰

적 요

사 사

키워드

Rice Ac/Ds Trait variation Herbicide resistance GUS