보리 생육환경 및 전처리 방법별 약배양 효율

• 영문명: Anther Culture Efficiency affected by Growth Condition and Pre-treatment Methods in Barley
• 발행기관: 한국육종학회
• 저자명: 박태일(Tae Il Park) 김영진(Young Jin Kim) 정선옥(Sun Ok Jeoung) 김현순(Hyun Soon Kim) 서재환(Jae Hwan Seo) 윤성중(Song Joong Yun)
• 간행물 정보: 『한국육종학회지』Vol.40 No.1, 31~38쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.03.30

영문 초록

This experiment was carried out to improve the anther culture efficiency of barley (Hordeum vulgare L.). Callus induction rates from anther cultures of the five domestic naked barley and four unhulled varieties ranged from 0 to 5.6%, and plant regeneration rate to callus was 30.4% in the donor plants grown in a greenhouse during winter, among which the green plant regeneration rates ranged from 0 to 4.4%. Plant regeneration rate was 30.4% in the donor plants grown in a greenhouse during winter, whereas 21.3% in the normal field condition in spring. In addition, callus induction rates were 19.2% in plants grown in a normal field and 7.2% in drought-stressed condition, respectively. Being Considered the anther culture efficiency affected by the sampling time, the optimum sampling stage of anthers was 3～4 days before heading when the length between the 1st and 2nd auricles reaches 5 to 10 cm and at the uninucleate of pollen which the tip of the 2nd auricle aligns with the middle of panicle in the leaf sheath. Best callus induction rates came from the anthers stored at 4℃ for 3 weeks in a 10 to 15 cm diameter polyethylene bag with 5 to 10 panicles and Duwonchapssalbori and Saessalbori showed the higher induction rate of 4.8% and 1.7%, respectively.

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서 언

재료 및 방법

결과 및 고찰

적 요

키워드

barley anther culture donor plant pre-treatment plant regeneration