꿀풀 추출물이 돌연변이원인 수은독성으로 손상된 배양 NIH3T3 섬유모세포에 미치는 보호 효과

• 영문명: Protective Effect of Prunella vulgaris L. var lilacina Nakai Extract on Cultured NIH3T3 Fibroblasts Damaged by Mutagenic Mercury-Induced Toxicity
• 발행기관: 인간식물환경학회
• 저자명: 오영희(Young Hee Oh) 박승택(Seung Taeck Park)
• 간행물 정보: 『인간식물환경학회지(JPPE)』제18권 제1호, 41~46쪽, 전체 6쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2015.02.28

영문 초록

This study was to evaluate the cytotoxicity of mercury, mutagene and the protective effect of Prunella vulgaris L. var lilacina Nakai extract (PVE) on methylmercuric chloride (MMC)-induced cytotoxicity in the cultured NIH3T3 fibroblasts. For this study, cell viability was measured after NIH3T3 fibroblasts were treated with 15~35uM concentrations of MMC for 72hours, respectively, and also, both of superoxide dismutase (SOD)-like activity and inhibitory activity of lipid peroxidation (LP) were assessed. In this study, MMC decreased cell viability in a dose-dependent manner, and vitamin E significantly increased cell viability which decreased by MMC-induced cytotoxicity in these cultures. In the protective effect of PVE, PVE prevented MMC-induced cytotoxicity by a significant increase of cell viability as well as SOD-like activity and inhibitory activity of LP. From these results, it is suggested that the oxidative stress is involved in the cytotoxicity of MMC, and also PVE showed the protective effect on MMC-induced cytotoxicity via antioxidative effect such as SOD-like activity and inhibitory activity of LP. Conclusively, the natural component as PVE may be a putative resources for the detoxification of heavy metals associated with the oxidative stress.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 결과 및 고찰

Ⅳ. 적요

Ⅴ. References

키워드

Antioxidative effect Lipid peroxidation Methylmercuric chloride