환경오염원인 크롬의 세포독성에 대한 청미래덩굴추출물의 보호효과

• 영문명: Protective Effect of Smilax china L. Extract on the Cytotoxicity Induced by Chromium of Environmental Pollutant
• 발행기관: 인간식물환경학회
• 저자명: 오용열(Yong Leol Oh)
• 간행물 정보: 『인간식물환경학회지(JPPE)』제14권 제1호, 29~34쪽, 전체 6쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2011.02.28

영문 초록

The purpose of this study is to evaluate the cytotoxicity of chromium and protective effect of Smilax china L.(SC) extract on glucose oxidase(GO)-induced cytotoxicity in the cultured C6 glioma cells. For this study, C6 glioma cells were treated in the media containing 15~30uM concentrations of chromium trioxide(CrO₃) for 72 hours, and cell viability and lactate dehydrogenase(LDH) activity with 1,1-diphenyl-2-picrylhydrazyl(DPPH)-radical scavenging activity were assessed on these cultures. In this study, CrO₃ decreased cell viability according to the treated dosage compared with control, and than the XTT₅₀ value was determined at 30uM CrO₃ in these cultures. In the protective effect of SC extract on CrO₃-induced cytotoxicity, both of an antioxidant, catalase and SC extract significantly increased cell viability which was decreased by CrO₃-induced cytotoxicity. SC extract also showed the antioxidative effect such as DPPH-radical scaveng activity and the decreased LDH activity. From these results, it is suggested that the cytotoxicity of chromium like CrO₃ is correlated with oxidative stress of reactive oxygen species(ROS), and also SC belong to Liliaceae showed the protective effect on the chromium-induced cytotoxicity by antioxidative effect in these cultures.

목차

Abstract

Ⅰ. 서론

Ⅱ. 재료 및 방법

Ⅲ. 결과 및 고찰

Ⅳ. 적요

Ⅴ. 인용문헌

키워드

cell viability chromium trioxide fee radical scavenging activity