배 ‘원황’(Pyrus pyrifolia) 유전체 해독에 기반한 SSR 마커 개발 및 유전자 지도 작성

• 영문명: Construction of a Genetic Map using the SSR Markers Derived from “Wonwhang” of Pyrus pyrifolia
• 발행기관: 한국육종학회
• 저자명: 이지윤(Ji Yun Lee) 서미숙(Mi-Suk Seo) 원소윤(So Youn Won) 임경아(Kyoung Ah Lim) 신일섭(Il Sheob Shin) 최동수(Dongsu Choi) 김정선(Jung Sun Kim)
• 간행물 정보: 『한국육종학회지』Vol.50 No.4, 434~441쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.12.30

영문 초록

High-density genetic linkage mapping is critical for undertaking marker-assisted selection and confirming quantitative trait loci, as well as helping to build pseudomolecules of genomes. We constructed a genetic map using 94 F1 populations generated from the interspecific cross between Korean cultivar “Wonwhang” (Pyrus pyrifolia, NCBI BioSample SAMN05196235) and European cultivar “Bartlett” (Pyrus communis). We designed a total of 24,267 SSR markers based on the genome sequences of “Wonwhang” for this. To select the markers that are linked to the traits important in pear breeding programs, SSR-containing genomic sequences were subjected to nucleotide sequence homology searches, which resulted in 510 SSR markers with high similarity to genes encoding proteins with putative functions such as transcription factors, resistance proteins, flowering time, and regulatory genes. Of these, 70 markers showed polymorphisms in parents and segregating populations and were used to construct a genetic linkage map, together with the unpublished 579 SNPs obtained from genotyping by sequencing analysis. The genetic linkage map covered 3,784.2 cM and the average distance between adjacent markers was 5.8 cM. Seventy SSR markers were distributed across 17 chromosomes with more than one locus.

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키워드

Pear Pyrus pyrifolia SSR marker Linkage map