Hepatoprotective Effect of Solvent Fractions from Raphiolepis indica against Oxidative Stress

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: Sun Young Kim Hye Ran Kim Kyung Soo Chang
• 간행물 정보: 『한국응용과학기술학회지』한국유화학회지 제35권 제4호, 1269~1275쪽, 전체 7쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2018.12.30

영문 초록

Raphiolepis indica (R. indica) is one of evergreen shrubs belonging to the Rosaceae and is grown wildly in Jeju. This study was performed to evaluate the hepatoprotective effect of different fractions (n-hexane, dichloromethane, ethyl acetate, butanol, water) from R. indica. Anti-oxidative effects were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and total phenol contents. Hepatoprotective effect was identified by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay in Huh7 cells. Among various fractions, ethyl acetate and butanol fractions showed the lowest DPPH remained rate reaching approximately 78.7 and 65.5% at 400 μg/mL. Ethyl acetate and butanol fractions showed the total phenolic content at 164.5 and 137.3 mg GAE/g extract. The ethyl acetate and butanol fractions were resistant against oxidative stress in MTT assay and showed higher hepatoprotective effect than other fractions. Therefore, these results suggest that the ethyl acetate and butanol fractions of R. indica might have therapeutic value in liver damage.

목차

Abstract

1. Introduction

2. Materials and Methods

3. Results and Discussion

4. Conclusion

References

키워드

Anti-oxidative effect Hepatoprotective effect Jeju natural extract Raphiolepis indica