Brassica 種들의 花뢰크기에 의한 小胞子 發育段階 및 胚發生 比較

• 영문명: Comparisons of developmental stages of microspore by bud size and embryogenesis from its microspore in Brassica species
• 발행기관: 한국육종학회
• 저자명: 장영석(Young Seok Jang) 민경수(Kyung Soo Min) 오용비(Yong Bee Oh) 정동희(Dong Hee Chung)
• 간행물 정보: 『한국육종학회지』Vol.29 No.4, 480~485쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.12.30

영문 초록

The purpose of this study is to search for the identifing method that could separate buds with embryogenic potential from collected buds for increasing the efficiency of embryogenesis by microspore culture in Brassica species. It was investigated developmental stage of microspore by bud-size and ratio of petal/anther length and compared to amount of embryogenesis through isolated microspore culture from the buds with late-uninucleate stage. In Brasica napus, Lisandra of spring type showed bud-size of 3.2~4.1mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/ anther length ratio were 1/2~3/4. Hallayuchae of winter type rapeseed showed bud-size of 3.1~3.6mm which belongs to late-uninucleate stage as embryogenic microspores and their range of petal/anther length ratio were 1/3~1/2. Two genotypes of B juncea had the same petal/anther length ratio as range of 1/2~3/4 with embryogenic microspores but their bud-size were some difference as bud-size of Young-sanpohwanggyeja were 2.9~4.0mm and bud-size of Hwanggyeja were 2.9~4.1mm. Also, two genotypes of B. campestris had the same petal/anther length ratio as range of 2/3~3/4 with embryogenic microspores but bud-sizes were some difference as bud-size of Hyakusai were 2.9~3.4mm and bud-size of Sosongchae were 2.9~3.2mm. In B. oleracea, the petal/anther length ratio with embryogenic microspores according to bud-size were showed range of 1/2~2/3 in all of two genotypes and bud-size of Broccoli were 2.4~3.2mm and bud-size of Cauliflower were 2.9~3.7mm. In the embryo yield per 100,000 microspores by isolated microspore culture from the buds with embryogenic microspores, Young-sanpohwanggyeja of B. juncea were 47 embryos and Hwanggyeja of B. juncea were 23 embryos, B. campestris of Hyakusai were 367 and Sosongchae were 123, B. oleracea of Broccoli were 678 and Cauliflower were 562. However in B. napus was showed much differences by the growth habit, the spring type of Lisandra were producted 7,047 embryos of which higher about 9.2 times than those of winter type Hallayuchae.

목차

ABSTRACT

緖言

材料 및 方法

結果 및 考察

摘要

引用文獻

키워드

Brassica species Microspore culture Embryogenesis Microspore development Bud size