γ-線처리 감자 節莖 器內培養에서 誘起된 突然變異體의 疫病抵抗性 反應과 RAPD 분석

• 영문명: Reaction to late blight(Phytophthora infestans) and RAPD polymorphisms of patato mutants induced from in vitro culture of nodal stem by γ-ray irradiation
• 발행기관: 한국육종학회
• 저자명: Jae Sung Kim(金在成) In Chui Shin(申仁撤) Jeung Haing Oh(吳正行) Young Sun Park(朴英善) Yong Hwan Lee(李龍煥)
• 간행물 정보: 『한국육종학회지』Vol.28 No.3, 323~331쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1996.09.30

영문 초록

For the purpose of mutation induction and selection of the potato variants from in vitro culture of nodal stem by the Υ-ray irradiation, their reaction to the late blight of potato(Phytophthora infestans) and RAPD analysis were conducted with the potato cultivars Jopung, Dejima and Superior. 1. Incidene of variations such as chlorophyll deficiency, leaf color change, adventitious root, microtuber formation was found in the nodal stem cultures. The induction rates of the morphological mutants were ranged from 19.3 % to 30.9% in 30Gy irradiation of Υ-ray as compared with 7.2% in the nonirradiated culture. 2. The plants at the 1st clonal generation originated from nodal stem culture showed very poor growth in the field. The plants derived from Υ-ray irradiated nodal stem culture revealed the marked reduction in plant growth than that of nonirradiated one. 3. Incidence rates of patato variants such as dwarf type, chlorophyll deficiency, change of leaf colors and tuber types at the 2nd clonal generation were 4.5% in nonirradiated group but 30.3% in irradiated group. The incidence rate of morphological mutants was 6.9% in Dejima, 3.4% in Jopung, 3.3% in Superior under the nonirradiated condition, while it was 31.0% in Superior, 30.7% in Jopung, 28.6% in Dejima under the irradiated condition. 4. The reaction of the potato variants selected from the 2nd clonal generation against late blight disease was different according to the original cultivars. The percent of diseased leaf area of selected potato variants from the Υ-irradiated population was significantly lower with the range of 18.8~41.3% according to the original cultivars as compared with those selected from the nonirradiated population. This suggested that there are some possiblity to select potato variants resistant to late blight by Υ-ray irradiation under in vitro culture condition. 5. In RAPD analysis of the potato plant originated from the 2nd clonal generation, five among twelve tested primers showed a good amplification and polymorphisms in twelve among 73 bands. The analysis sugguested that the homogenity between Dejima and Superior was closer than that between Superior and Jopung. The selected resistant potato variants exhibited considerably different banding patterns in two primers.

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키워드

Radiation Mutation Potato Late blight resistance