Detecting DNA markers by RFLP analysis and DNA-fingerprinting in capsicum

• 발행기관: 한국육종학회
• 저자명: Dong Young Shin(申東永) Hak Soon Choi(崔學淳) Hyo Yeon Lee(李孝淵) Kwon Kyu Kang(姜權圭) Ill Sup Nou(盧一燮)
• 간행물 정보: 『한국육종학회지』Vol.28 No.2, 205~212쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1996.06.30

영문 초록

This experiment was carried out to detect of cultivar specific markers using RFLP analysis and DNA-fingerprinting in Capsicum genus. The result of SDS-PAGE analysis could not detect genotype specific protein markers workable within each species of Capsicum, it however was possible to detect some interspecies markers. For RFLP analysis and DNA-fingerprinting, DIG labelled (GATA)₄, 26S rRNA, rice chloroplast DNA clones (P1, P2, P10-1, P45, P50, B3), and maize mitochondrial DNA clone (ATP A) were used as a probe. With a (GATA)₄ probe the level of polymorphism detected was high enough to differentiate all 29 genotypes used in this study. The results with the most informative banding patterns were obtained with the (GATA)₄ probe hybridized to Hae Ⅲ, Rsa Ⅰ, and 26S rRNA probe hybridized to BamH Ⅰ, Hinf Ⅰ, Hae Ⅲ, Hind Ⅲ resticted genomic DNA, respectively. Hybridization with the P2 probe which produced polymorphic bands (11kb) in only Masanjere by HindⅢ digest. Mitochondrial ATP A probes revealed three variable band patterns among the 29 red pepper genotypes digested by Hind Ⅲ and HaeⅢ. A same band patterns was obtained between X-mas bell Ⅰ and X-mas bell Ⅱ on a HindⅢ digest. In case of Green 100 genotype specific band detcted by HindⅢ, HaeⅢ digested. Subsequently, the multilocus probe, (GATA)₄, 26S rRNA and ATP A probes has proven successful in distingulishing between different red pepper genotypes.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS AND DISCUSSION

摘要

Literature Cited

키워드

SDS-PAGE RFLP DNA-fingerprinting (GATA) 26S rRNA ATP A cpDNA clone mtDNA clone Capsicum