Chromosomal location of tillering gene in maize inbred line, Pl 213749

• 발행기관: 한국육종학회
• 저자명: Hee Bong Lee(李喜鳳) E.H. Coe Bong Ho Choe(崔鳳鎬)
• 간행물 정보: 『한국육종학회지』Vol.28 No.2, 142~145쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1996.06.30

영문 초록

In order of determine the chromosomal location of additional tillering gene, the PI213749 inbred was crossed with a non-tillered inbred, KV₁/IK₁, which has been selfed for six generations, and F₂ kernels were produced for RFLP mapping. Leaf tissue was collected from F₂ individuals and parents. After freeze drying, genomic DNA was extracted using the CTAB method of UMC Maize RFLP Laboratory Manual. DNA was digested with EcoRⅠ, HindⅢ and Dra I. DNA of the seperated parental lines, non-tiller and three or more tiller individuals, was loaded onto 0.8% agarose gels and electrophoresed. DNA was transferred to Magnacharge membrane according to standard laboratory protocols. Hybridization using dCTP³² oligolabelled probes from the Maize Core Marker Set indicated that tillering is linked to umc76 on the short arm of chromosome 1. Additional probings to establish whether the locus is proximal or distal to umc76 are in progress. Future testing will be needed to determine whether the gene from PI213749 and tlr I of Neuffer are allelic.

목차

ABSTRACT

INTRODUCTION

Materials and Methods

Results and Discussion

摘要

References

키워드

Chromosome Tiller RFLP map genomic DNA Probe Proximal. Distal Agarose gel Electrophoresis Hybridization Allelic Maize Core Maker