들깨 葯培養에서 低溫 및 生長調整劑 處理가 캘러스誘導와 植物體 再分化에 미치는 影響

• 영문명: Effect of low temperature and plant growth regulators on callus induction and plant regeneration in anther culture of perilla
• 발행기관: 한국육종학회
• 저자명: Hyo Sung Lee(李孝承) Jung Il Lee(李正日) Su Noh Ryu(柳守魯) Han Sun Hur(許翰淳)
• 간행물 정보: 『한국육종학회지』Vol.26 No.4, 345~352쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1994.12.30

영문 초록

This experiment was carried out to determine the effect of low temperature pre treatment and plant growth regulators on callus induction and plant regeneration in perilla anther culture. Perilla panicle with floral envelope was pretreated in low temperature at 5℃ and l0℃ for three days and compared with contral. MS basic medium supplemented with 2, 4-D 1~5㎎/ℓ, NAA 1~3㎎/ℓ and kinetin 2~5㎎/ℓ was used for callus induction and MS medium supplemented with NAA 0.1㎎/ℓ and BA 0.5㎎/ℓ was used for plant regeneration. Cold treated anthers at 5℃ for three days induced more callus and plant regeneration than others. In percentage of callus induction, MS medium supplemented with 2, 4-D ㎎/ℓ, NAA 1㎎/ℓ and kinetin 2㎎/ℓ induced more callus than N6 and B5 medium supplemented with the same concentration of plant growth regulators. The percentage of plant regeneration of Yeopsil×Jukjaso (F₁) in MS medium supplemented with 2, 4-D 5㎎/ℓ, NAA 3㎎/ℓ and kinetin 5㎎/ℓ was about 63%, while that in MS medium supplemented with 2, 4-D 2㎎/ℓ, NAA 1㎎/ℓ and kinetin 2㎎/ℓ was about 32%. The percentage of plant regeneration of suwon 32 showed the same tendency at the same condition in the case of 10℃ pretreatment for three days on Yeopsil×Jukjaso(F₁).

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材料 및 方法

結果 및 考察

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키워드

Perilla Anther culture Cold pretreatment Plant growth regulator Plant regeneration Callus induction